FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005183198

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005183198
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15631936
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGTGGT90550.030.5334054
AGTGGTA104250.026.8661865
AGCAGTG118950.023.6670862
TGGTATC129150.021.7979097
GGTATCA131850.021.2423218
GTGGTAT135850.020.7758566
GTATCAA140750.020.1548849
GCAGTGG143500.019.5679443
AAGCAGT162300.017.7449151
ACTGAGT24300.011.2592591
TGAGTAG24900.010.6987943
AGAGCAC164250.09.1178088
GAGCACA159550.08.8448769
GATCGGA196150.07.34132051
CTGAGTA38300.07.1436032
TAGGAAG44550.06.7878797
TCGGAAG206650.06.61988933
TCTTGGG96100.06.203537535-39
ATCGGAA233400.06.2005142
GAGTAGG44150.06.1970564
Produced by FastQC (version 0.11.5)