FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005183199

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005183199
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15631936
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCTCA114900.019.1122726
CTCAAAC127950.017.4443139
GGCTCAA125700.017.1837717
ATGGCTC127300.016.9677935
TCTGATG136850.016.3098281
GATGGCT161150.013.850454
ACTGAGT23800.012.1008411
GCTCAAA187350.011.95196158
TGAGTAG27650.010.67631153
CTGATGG263100.08.6750282
TGATGGC284300.08.6106223
CCTCTAG16050.07.80560850-54
CTGAGTA37900.07.78891852
CTCGGGT60750.07.60888920-24
GATCGGA198400.07.25806471
TCGGAAG210950.06.9969183
AGAGCGT208000.06.8192318
ATCGGAA233050.06.79682452
TAGGAAG47850.06.77115967
GAGTAGG46750.06.622464
Produced by FastQC (version 0.11.5)