FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005183221

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005183221
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18934662
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGCTC128350.012.8431365
GGCTCAA129700.012.5984587
TGGCTCA138250.012.2879196
CTCAAAC151250.011.088999
GATGGCT162950.010.2044654
TCTGATG168050.09.4268151
CCTCTAG13900.08.49441550-54
GCTCAAA228950.07.5457448
TGATGGC299900.06.3608763
CTCTAGG11000.06.283230350-54
CTGATGG278250.06.02871132
TCTAGGA12850.05.60275150-54
CTAGGAT13750.05.34074650-54
CCCTAAG18350.0086360255.09963136
Produced by FastQC (version 0.11.5)