FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005183225

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005183225
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14146067
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCTCA99000.019.922066
ATGGCTC100350.019.2952635
GGCTCAA104400.019.0985187
TCTGATG119400.016.3490561
GATGGCT126900.016.1090894
CTCAAAC137500.014.5534379
CCTCTAG11050.011.33449550-54
GCTCAAA194000.010.3890718
CTCTAGG8050.010.37235350-54
CTGATGG195200.010.3256042
TGATGGC216000.09.8645393
TCTAGGA12250.07.403714750-54
CTCGGGT58000.07.173422320-24
CTAGGAT11700.07.136533350-54
AACACAG75250.06.122202410-14
GGTCCTC72300.05.9536545-49
CTAGCAC8950.05.951464750-54
AGGTCCT74900.05.881525545-49
GGGTGGG74250.05.661737425-29
ACACAGC86900.05.4505510-14
Produced by FastQC (version 0.11.5)