FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005183236

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005183236
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12680540
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTC347170.27378171592061534No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA113100.020.116279
AGAGCAC117450.019.3099238
GATCGGA123000.018.8027341
TCGGAAG179850.012.8161053
AAGAGCA184800.012.7400317
ATCGGAA197400.012.1521472
GAAGAGC213050.010.8817576
GGAAGAG258750.09.4885255
CGGAAGA283450.08.1790734
CTCGTAT74500.06.359225740-44
CGTGAGA77850.06.159791535-39
CAGTCAC77900.06.137037325-29
CACGTCT81900.06.100965510-14
AGTCACC83250.06.002101425-29
TCTCGTA75450.05.973786440-44
CACACGT83950.05.866220510-14
ACACGTC88150.05.701066510-14
CCAGTCA92000.05.431249625-29
AATCTCG84950.05.35658940-44
ACTCCAG100900.05.023489520-24
Produced by FastQC (version 0.11.5)