FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005183237

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005183237
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12680540
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAG179010.1411690669324808No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCTAACTCGTGTAGATCT133800.10551601114779023Illumina Single End PCR Primer 1 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA124700.018.3648051
AGAGCGT148650.015.6447038
GAGCGTC159750.014.6027249
AAGAGCG170550.013.6780157
TCGGAAG176000.013.4180973
ATCGGAA197500.012.3220442
TAACTCG42600.010.715425535-39
GAAGAGC221000.010.6859056
CTAACTC43550.010.34941830-34
GCTAACT43450.010.340095530-34
TAGATCT47950.09.670011540-44
TGCTAAC51000.09.28935230-34
GTGCTAA52700.08.88039930-34
GGAAGAG265200.08.8506225
ACTCGTG56200.08.68606735-39
CTCGTGT55750.08.49788335-39
CGGAAGA296500.08.1348554
TGTGCTA63100.07.553675730-34
GTAGATC67950.07.16286940-44
AGATCTC72050.06.77525245-49
Produced by FastQC (version 0.11.5)