FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005183250

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005183250
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16852917
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACTA51550.019.5510438
CTACACT56700.017.1404117
ACACTAT65600.015.2539219
ACTACAC82150.012.4437516
CCGTTAA74550.011.9739324
ACAACTA91800.011.3006883
CTAGCTC20350.010.25981935-39
CGTTAAA86950.09.7697685
AACTACA111000.09.6635155
TACAACT104700.09.56432
ATACAAC115300.09.213871
TCCGTTA112550.08.2571333
GTTAAAT105200.08.0064626
TCTAGCT26150.07.984218635-39
CAACTAC137100.07.6136584
CTCTAGC32800.06.804464335-39
CCCTAAG33900.06.413754125-129
CATTAGG37650.06.1960654100-104
TCTCTAG33550.06.1802535-39
CTATAGC39600.05.853626710-14
Produced by FastQC (version 0.11.5)