FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005183253

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005183253
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18877373
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCACTCA51400.014.5663649
GCCACTC75200.010.3391988
AGCCACT96350.08.2190347
AAGCCAC109550.07.8861046
AACACTA64500.07.7013917
ACACTAT78050.06.7333458
CCCTTAA80300.06.72437574
AATTACG104850.06.53049471
CTCACTA30150.05.96913115-19
CACTATA89750.05.69514569
TACAAAG181400.05.443292
ACAAAGC188850.05.1850433
GGCCTAA35100.05.12781675-79
GTTCCCT133800.05.00862361
Produced by FastQC (version 0.11.5)