FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005183265

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005183265
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16760735
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGAGTA41700.010.708822
TAAGGGG42700.09.9479579
TAGCATG36700.09.8085758
AGTGAGT43350.09.4752531
GTGAGTG52900.07.7646931
TGAGTGA68650.06.1900972
GTCCCTT79350.05.90298271
GAGTGAT60750.05.8072073
TGAGTAT96150.05.69091133
CCTTTAG65950.05.45837834
GTATAAG87750.05.41506866
GAGTATA90250.05.34485634
ATAAGGG85800.05.2863428
TTAGCAT72750.05.14603047
Produced by FastQC (version 0.11.5)