FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005183266

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005183266
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13922159
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGAGTG40000.024.5430811
TGAGTGA53250.018.6773762
GAGTGAT57150.017.907173
CTTCCTA56400.016.72294
TTAGTCC25350.016.1881649
CTTAGTC25900.016.1223728
AGTGATG80850.013.0905144
TCCTATT86350.011.3392286
CCTTAGT37200.011.2249857
ATGGTTG100450.010.4641428
CCTATTT94750.010.1058577
TGGTTGA107550.09.8402839
TTCCTAT104950.09.6040095
TAGTGTG23600.09.21285215-19
TCCTTAG52200.09.1029136
GATGGTT128100.08.5989027
ATGCTTC144800.06.7798571
CTATTTT177200.06.0943628
GTAGTGT39050.05.899674415-19
CTCCTTA86300.05.8397495
Produced by FastQC (version 0.11.5)