FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005183276

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005183276
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13494950
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACCACTGTATCTCGTAT145860.10808487619442828TruSeq Adapter, Index 1 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTGTC50600.027.766142
GGTGTGT64050.024.5477241
TGTGTCA77450.018.4193883
GTGTCAT96800.014.5013734
CATAAGT102950.013.3554988
ATAAGTC116200.012.5760149
GTCATAA124450.011.4531086
GGTGTCC73500.010.0025481
GTGTCCA63700.09.8403652
TGTCATA150000.09.8382385
TCATAAG148250.09.7601037
GGTGTTT97300.09.575771
GGTGTTG152550.09.35234451
GGTGTAA106450.08.6159161
GGTATAG95800.08.35802651
GGTGTGA83650.08.266741
GGTGTAG118700.08.033361
GGTGTAT90850.07.8519541
GGTATAT128450.07.53692341
GGTATAA129600.07.52621131
Produced by FastQC (version 0.11.5)