FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005183277

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005183277
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13494950
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCATAGCCGGTGTAGATCT154090.11418345381049948Illumina Single End PCR Primer 1 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTGTC51200.025.6197642
GGTGTGT64100.023.4068971
TGTGTCA77700.017.142223
CATAAGT105050.012.1299278
ATAAGTC114950.011.9620879
GGTGTCC75850.011.1387661
GTCATAA127350.010.6846796
GTGTCCA65400.09.6979212
GGTGTAA108700.09.44765951
TCATAAG149150.08.9777817
GGTGTTG150150.08.7798671
GGTGTTT97950.08.2537861
GGTGTTC133550.08.0714671
GGTGTCT73750.07.9994041
GGTGTAT89350.07.74397131
GGTATAC49850.07.74364381
GGTGTAC73900.07.6874951
GGTAGTG70250.07.67220261
GGTATCT125650.07.3616621
GGTGTTA62850.07.18490651
Produced by FastQC (version 0.11.5)