FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005183364

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005183364
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15441233
Sequences flagged as poor quality0
Sequence length150
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCCTA22450.09.6277644
CTTAGTC15854.731828E-77.72071468
GTTACAC27101.4188117E-106.90624337
TTAGTCC16053.6113648E-56.7275059
CCCTACT35150.06.5533366
CCTTAGT18001.7565837E-45.9986927
TCAGTTA28407.380513E-75.5811654
TCCTTAG25001.6278895E-55.47080766
TTACACA37105.7862053E-95.4327788
AGTTACA33651.1848533E-75.34801676
CCTACTT47502.3646862E-115.3041077
TCCCTAC49106.184564E-115.1312645
Produced by FastQC (version 0.11.5)