FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005183372

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005183372
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17522097
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATACG72200.06.7759361
GTATACT63100.06.59594731
GTATATC131500.06.55221181
GTATAGG62850.06.15746931
CTACGTA28351.834087E-86.09615043
GTATATT155750.05.8602161
GTATAGC81150.05.8486621
GTATAGT65000.05.84146361
GTATGCT101450.05.82994751
TGAGTAG32207.4578566E-95.8145343
GTATATG115750.05.67744871
CTTAGTC24209.423438E-65.65206058
GTATAAG85750.05.62006761
GTATAGA78350.05.49850561
TTAGTCC22401.0165596E-45.46346579
AGTGAGT40455.9426384E-95.2349341
CTCCCTA30502.9312378E-65.1926636
GTATGAG91450.05.1899281
GTATCCT111050.05.1287051
GTATCCA110150.05.104321
Produced by FastQC (version 0.11.5)