FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005183406

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005183406
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14408640
Sequences flagged as poor quality0
Sequence length150
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTC827880.5745719235125591No Hit
AGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAG251220.17435372110067293No Hit
GAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGA155530.10794217913696227No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA165750.015.2874739
AGAGCAC180350.014.169648
GATCGGA198550.013.2218931
TCGGAAG207950.012.3011943
AAGAGCA234300.011.1219997
ATCGGAA238500.011.0578462
GAAGAGC300800.08.8067676
GGAAGAG326550.08.1343575
CGGAAGA328400.08.0527134
AGTCACA73050.06.997640625-29
GGGGGGG56450.06.25475032
CTCGTAT86050.06.006548440-44
CAGTCAC92650.05.98354825-29
TCGCAAT99600.05.695247735-39
CCAGTCA98350.05.46107325-29
GTCACAG106800.05.244714725-29
CACACGT102250.05.23792410-14
TCGTATG99050.05.145498845-49
TCTCGTA102050.05.10713140-44
CACGTCT105200.05.09104310-14
Produced by FastQC (version 0.11.5)