FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005183407

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005183407
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14408640
Sequences flagged as poor quality0
Sequence length150
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAG488530.33905351233704223No Hit
GAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGA343740.23856519421680322No Hit
AAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAA249750.17333349990005997No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA202150.012.7804951
TCGGAAG213850.012.2351123
AGAGCGT217300.011.3293228
ATCGGAA237700.011.198882
GAGCGTC246150.010.2646659
AAGAGCG252150.09.706457
GAAGAGC320700.08.1479466
GGAAGAG332450.07.83831555
CGGAAGA333450.07.82507324
TAGATCT79700.06.720256340-44
TCATTAA80000.06.640883465-69
TGGGGGG43750.06.0395311
GTGCACT90400.05.84486530-34
AGAGTGT93250.05.836028625-29
GGGGGGT28156.072132E-75.6379562
GAGTGTG94850.05.509899625-29
TAGGGAA100850.05.467777715-19
ATCATTA103800.05.451360-64
GTGTAGA107350.05.351457640-44
CGTATCA111200.05.347491760-64
Produced by FastQC (version 0.11.5)