FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005183413

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005183413
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19929696
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACTATA67900.025.1299869
AACACTA74450.023.0152727
ACACTAT79950.021.4319828
AAACACT121700.015.0854726
CAAACAC130600.013.616465
ACCAAAC133000.013.0461493
CCAAACA137600.012.8191164
TATTTAG94300.08.3983639
GTAGCCT101350.07.9639341
TACCAAA259300.07.4445572
ATACCAA305100.06.80273681
GCCTTTA133900.06.50601674
TTAGGCA64950.05.91921160-64
TACACTC65650.05.8127146115-119
ACTATAG67900.05.55614910-14
CTATAGT70300.05.5508110-14
GTGAGTG56100.05.26689151
TTTAGGC73750.05.23244260-64
TAGCCTT159100.05.20636032
GTACACT74100.05.1498227110-114
Produced by FastQC (version 0.11.5)