FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005183458

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005183458
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16648488
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATAGCCACTCAACTCACTATATAGCCACTATACCATATAGCTCTACTA194960.11710372737752522No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCACTCA68550.042.9515957
GCCACTC80800.036.7982256
TAGCCAC85850.034.5513234
ACTCAAC87750.034.1281139
CACTCAA88800.033.4813658
AGCCACT127800.023.7166775
TATAGCC132400.023.1104662
CCGTTAA72100.022.8669384
ATAGCCA145150.020.7828333
TAAGGGG57300.018.970929
CGTTAAA87000.018.9504185
TCCGTTA100950.016.9737743
ATATAGC191650.016.3878971
GTTAAAT133300.013.0699366
AGTAAGT85000.012.744291
CTCACTA51100.012.40053115-19
TAGCATG48200.011.9483798
GTTCCGT154300.011.2139671
GTAAGTA103000.011.1139312
CCCCTAA59500.010.671244110-114
Produced by FastQC (version 0.11.5)