FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005183466

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005183466
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15784474
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACGTA41400.012.1759543
AGGCCTC42350.010.5392927
GCTACGT49050.010.2769522
AGCTACG59200.09.2489051
CAGGCCT62450.07.60824786
TACGTAA79400.06.89284234
AGATCCA78450.06.24006567
GGCCTCC80300.06.18597468
AGAGCAC149900.06.0992538
GAGCACA152550.05.99331959
ACAGATC99300.05.87230975
CCCACAG99400.05.6508462
GATCCAT97900.05.29449278
GATCGGA174250.05.0694791
CAGATCC119900.05.04351476
Produced by FastQC (version 0.11.5)