FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005183480

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005183480
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11425223
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACAAAGCCACTCAACTCACTATATAGCCACTATACCATATAGCTCTAC127150.11128885624376872No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCACTCA56900.035.172269
GCCACTC61300.032.647668
AAGCCAC91600.021.9278226
ACGTTAA51600.021.0683774
AGCCACT98600.020.443027
GTTACGT59150.018.9288221
TACGTTA60900.018.560273
CGTTAAA75550.017.8197445
ACAAAGC134500.015.5765993
TACAAAG148850.014.8004382
GTTAAAT94850.013.8140426
GGCCTAA32950.012.71573375-79
TTACGTT96850.012.2655292
ATACAAA195000.011.6326231
AAAGCCA191800.010.922925
CTCACTA40500.010.41878715-19
GGGCCCC38500.09.573596110-114
GGGGCCC39300.09.342331105-109
CAAAGCC231450.09.1763234
GGCCCCT47050.09.08851110-114
Produced by FastQC (version 0.11.5)