FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005183481

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005183481
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11425223
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACAAAGCCACTCAACTCACTATATAGCCACTATACCATATAGCTCTAC125560.10989719850544712No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCACTCA52650.034.4508139
GCCACTC58850.030.7042668
AAGCCAC92900.019.4663836
AGCCACT96650.018.923457
ACGTTAA54700.018.4412694
GTTACGT60000.018.0920871
CGTTAAA72000.017.3119755
TACGTTA60850.016.8142643
GTTAAAT93100.014.2395226
ACAAAGC143650.013.1916833
TACAAAG149500.012.628622
GGCCTAA32700.011.71419875-79
TTACGTT94750.011.33190252
CTCACTA37100.010.40207115-19
ATACAAA199150.09.8335221
AAAGCCA200500.09.4509265
ACTCACT43350.09.40061515-19
GGGGCCC39000.08.861558105-109
GGGCCCC38700.08.746443110-114
GGCCCCT45850.08.513429110-114
Produced by FastQC (version 0.11.5)