FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005183486

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005183486
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12125395
Sequences flagged as poor quality0
Sequence length150
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGACTA40400.07.95882841
ACTACTC42500.07.45306874
AGGCTCC91850.07.446035
GGCTCCA97000.07.1990176
AAGGCTC101650.06.72802544
TGACTAC44000.06.71000152
TCCAGAC114600.06.3446839
CTCCAGA111650.06.31888638
AGAAAGG124600.06.2754311
GACTACT52300.06.0581833
TACTCCC59650.05.79300556
GGCCCTA16700.00284013325.60555033
AAAGGCT133750.05.5992643
ACTCCCC62850.05.1544277
GAAAGGC160700.05.15315682
Produced by FastQC (version 0.11.5)