FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005183520

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005183520
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14963937
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGGTA67550.022.5973475
CAGTGGT66950.022.271364
AGCAGTG101150.015.8068152
GTGGTAT103550.015.018636
GCAGTGG118750.012.920313
GTATCAA123200.012.9157999
GGTATCA125750.012.6535078
TGGTATC126150.012.0997197
AAGCAGT147700.010.6342081
Produced by FastQC (version 0.11.5)