FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005183539

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005183539
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15992277
Sequences flagged as poor quality0
Sequence length150
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCTCA119150.012.5668036
CTCAAAC120500.012.5455359
GGCTCAA119250.012.31483657
ATGGCTC120650.012.2313765
TCTGATG160600.09.8705131
GATGGCT169500.08.791284
GCTCAAA173400.08.7182068
CCTCTAG10650.08.6519650-54
CTCTAGG7350.07.247672650-54
TCTAGGA8600.07.19870250-54
CTAGGAT10800.06.398845750-54
CTGATGG273850.06.07254932
TGATGGC315750.05.6084553
CTCGGGT55050.05.15217920-24
CTAGCAC7554.8896254E-85.148740350-54
Produced by FastQC (version 0.11.5)