FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005183552

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005183552
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18488235
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTC315280.17053006952799984No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATGAATCATCTCGTAT221760.11994654979234091TruSeq Adapter, Index 9 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACGTA42950.054.9945263
TACGTAA55050.042.9067154
GCTACGT68700.034.171942
ACGTAAA93700.025.6612745
AGCTACG92850.025.392381
AGGCCTC116550.020.074397
GATCGGA230400.016.7909871
CAGGCCT142150.016.7630276
AGAGCAC229050.016.6892018
GAGCACA231150.016.4441039
CGTAAAA161100.015.2380766
TCGGAAG253450.015.1441073
ATCGGAA284700.014.0129982
AAGAGCA319700.012.1146527
CCACAGA136400.012.0900993
TCCTCAG155350.012.0021399
GATCCAT139350.011.9337338
TACGTAG39200.011.754127140-144
GCCTCCA222650.011.025599
GAAGAGC363450.010.6959766
Produced by FastQC (version 0.11.5)