FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005183553

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005183553
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18488235
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGCGTAGCGTGTAGATCT233270.12617213054680448Illumina Single End PCR Primer 1 (97% over 34bp)
AGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAG228540.1236137467962734No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACGTA40750.056.238443
TACGTAA52900.044.4120064
GCTACGT68400.033.287872
AGCTACG89850.026.015191
ACGTAAA95950.024.4104445
AGGCCTC118900.019.7593147
CAGGCCT139850.016.9538046
GATCGGA231250.016.2551161
TCGGAAG254300.014.93475153
CGTAAAA165800.014.7784246
AGAGCGT264400.013.9827338
ATCGGAA291750.013.3857732
GAGCGTC291150.012.8606199
CCACAGA138600.012.6870423
AAGAGCG298600.012.6467117
GATCCAT137350.012.64516458
TACGTAG38800.012.393551140-144
TCCTCAG153200.011.7035039
GCCTCCA217200.011.0397629
CCCACAG164950.010.9205042
Produced by FastQC (version 0.11.5)