FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005183565

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005183565
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12619955
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGCTC87450.015.0643055
TGGCTCA94300.013.9700276
GGCTCAA98250.013.8479467
TCTGATG98750.013.5948031
CTCAAAC106750.013.1499129
GATGGCT113100.011.7783834
CCTCTAG10400.08.99825350-54
GCTCAAA165450.08.6584958
TCTGAGG39850.08.6052161
CTCGGGT45100.08.17224320-24
CTCTAGG8550.07.91425350-54
CTGATGG189700.07.4062852
TGATGGC217800.06.74435233
TCTAGGA10450.06.613070550-54
CTAGGAT12050.05.854465550-54
GGTCCTC75050.05.275462645-49
AGGTCCT75650.05.157496545-49
TCGGGTG77950.05.09766725-29
AACACAG72150.05.028553510-14
Produced by FastQC (version 0.11.5)