FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005183568

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005183568
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11816503
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGTGGT60050.020.6214874
AGTGGTA67550.018.3315965
AGCAGTG81550.015.0978562
GTGGTAT103200.012.3474676
GCAGTGG100350.012.1966283
AAGCAGT109750.011.8851971
GTATCAA115950.011.4242019
GGTATCA122250.010.8356088
TGGTATC120550.010.8092537
AACACTA35400.08.33805757
CACTATA41100.08.2325589
ACACTAT39900.07.93896878
AAACACT60200.05.3814636
Produced by FastQC (version 0.11.5)