FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005183569

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005183569
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11816503
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGCTC88000.013.17042355
TGGCTCA86100.012.8757976
CTCAAAC92600.012.7493369
GGCTCAA90200.012.5299057
TCTGATG96100.011.1579191
AACACTA34500.010.0155967
CACTATA40400.09.2656679
ACACTAT39700.09.2477138
GATGGCT120550.09.1986084
GCTCAAA129450.09.1200358
CTGATGG163800.06.77277572
TGATGGC195400.06.3013393
AAACACT64650.05.67882166
CTCGGGT48050.05.003801320-24
Produced by FastQC (version 0.11.5)