FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005183592

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005183592
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16801303
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGGTA82900.020.842145
CAGTGGT89800.019.3208564
GTGGTAT129350.013.6915616
AGCAGTG132200.013.5091872
GCAGTGG145700.012.2073773
GTATCAA152250.012.1996339
TGGTATC151400.012.0303697
GGTATCA156300.011.3768568
AAGCAGT190600.09.64781951
ACTATAG32455.3934855E-85.546389
Produced by FastQC (version 0.11.5)