FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005183614

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005183614
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13599037
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGTGGT61800.013.0476294
CCCTTAG35100.011.0759445
CCCCTTA36450.09.6783344
AGCAGTG92550.09.102442
AGTGGTA89700.08.748395
GCAGTGG93500.08.4702433
TGGTATC98650.08.3193747
GGTATCA99700.08.3039678
GTGGTAT96450.08.2105716
GTATCAA117400.07.11332759
CCTTAGA54450.07.00746446
TTAGAGC49100.06.5980258
GTGCACA52900.06.5750521
CTTAGAG53950.06.40527
AAGCAGT153050.06.20228341
TAGAGCC55050.06.01566179
Produced by FastQC (version 0.11.5)