FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005183615

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005183615
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13599037
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTTAG32600.09.2744795
CCCCTTA35600.08.2924484
GTGCACA53600.07.6213021
GGCTCAA98250.07.2536887
ATGGCTC100900.07.13455155
TGGCTCA108400.06.84015276
CTCAAAC115300.06.6180929
TTAGAGC51400.06.44244868
CTTAGAG57550.05.75398547
CCTTAGA55650.05.6917446
TAGAGCC53650.05.63554339
GATGGCT137500.05.34129244
GCTCAAA150150.05.03408058
Produced by FastQC (version 0.11.5)