FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005183617

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005183617
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12839245
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA125650.010.9701171
AGAGCGT139850.09.9347048
GAGCGTC141750.09.8523259
GGCTCAA99950.09.0030017
TCGGAAG159400.08.7634823
AAGAGCG164500.08.7523397
TGGCTCA118900.07.4470646
ATGGCTC108900.07.4037535
GAAGAGC200400.07.39999256
CTCAAAC121000.07.37728269
CCAACAC70400.07.0556427
ATCGGAA220100.06.87370632
GATGGCT124900.06.86050844
TACCAAC72650.06.6389745
CAACACT71650.06.63113458
GGAAGAG233600.06.59481675
GCTCAAA143150.06.53750568
TAGATCT55800.06.037646340-44
CGGAAGA247250.05.94108254
TGATGGC176300.05.8404593
Produced by FastQC (version 0.11.5)