FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005183618

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005183618
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14894621
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACCAAACACTATAGCTCTCAAAATTTAAAGTACTAAATCCTTGAGGCC169370.11371219180400763No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACTATA67100.040.874249
AACACTA69200.040.673967
ACACTAT68850.040.4626438
AGTGGTA106400.032.7441335
CAGTGGT117650.029.6140464
GTGGTAT136550.025.8304026
AAACACT112550.025.2626236
AGCAGTG138200.025.0108092
CAAACAC121400.022.9467955
GCAGTGG157000.021.9240783
AAGCAGT162400.021.7898031
TGGTATC174550.020.5378637
ACCAAAC141700.020.4799213
GGTATCA181700.019.6505178
GTATCAA186950.019.5222439
CCAAACA152250.018.9596964
GAGCACA120000.016.556759
TACCAAA180950.016.2764322
ATACCAA206900.015.048081
AGAGCAC131100.015.0451018
Produced by FastQC (version 0.11.5)