FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005183619

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005183619
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14894621
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACCAAACACTATAGCTCTCAAAATTTAAAGTACTAAATCCTTGAGGCC179460.12048644943701486No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACTATA69050.040.450889
ACACTAT69850.039.9862448
AACACTA73450.038.126337
GGCTCAA116050.025.743657
ATGGCTC114900.025.565145
CTCAAAC122800.024.7385359
AAACACT116000.024.515866
TGGCTCA129750.022.861146
ACCAAAC141250.020.6937773
CCAAACA146600.019.5953394
CAAACAC146300.019.5368355
GCTCAAA162550.018.334068
GATGGCT173850.017.2279054
TCTGATG172450.017.018211
TACCAAA177700.016.4497132
TAGCCCT59550.015.1129052
ATACCAA203500.014.6368221
GATCGGA139000.014.1807771
GCCCTTA65650.013.7084744
CTGATGG260550.011.6611422
Produced by FastQC (version 0.11.5)