FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005183624

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005183624
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21126779
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGGTA116450.028.4957715
CAGTGGT122450.026.9911214
GTGGTAT158200.021.0665536
GCAGTGG173700.019.4531973
AGCAGTG177100.019.4053672
TGGTATC176450.019.2961567
GGTATCA191200.017.6193268
GTATCAA223300.015.2800599
AAGCAGT242700.014.2039151
GCAGAGT148950.05.151664715-19
TCTTGGG144750.05.044728335-39
Produced by FastQC (version 0.11.5)