FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005183641

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005183641
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17723710
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCACTCA43550.017.3570389
TGGCTCA114450.011.5109136
ATGGCTC113800.011.3236195
GGCTCAA117600.010.8965037
GCCACTC74500.010.3395598
AGTGAGT32100.09.3502941
AGCCACT85000.08.7235417
CTCAAAC152000.08.6672639
TCTGATG147000.08.61539651
GATGGCT165000.08.0281554
AAGCCAC105100.07.9456566
GTGAGTA37450.07.30486872
GCTCAAA201700.07.2097468
TAAGGGG47800.07.07855229
GGCCTAA24100.06.631479775-79
CTCACTA25300.06.146402415-19
TACAAAG149250.05.4988412
CTAGGAG12000.05.27938750-54
ATACAAA192150.05.0289811
Produced by FastQC (version 0.11.5)