FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005183643

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005183643
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16094923
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCTCA121200.020.6726386
GGCTCAA120350.020.3400557
ATGGCTC120600.020.1784925
CTCAAAC136400.018.5796189
TCTGATG162650.015.0803921
GATGGCT173650.014.6769894
GCTCAAA188650.013.5477318
CCTCTAG13700.012.71838450-54
CTCTAGG12250.011.04991350-54
CTGATGG243900.010.4880562
TGATGGC274800.09.8770613
TCTAGGA14900.09.85782250-54
CTCGGGT56700.08.96479620-24
CTAGGAT16800.08.48580550-54
GGTCCTC89500.06.24168545-49
AGGTCCT88300.06.081928345-49
GGGTGGG99350.05.753534325-29
TCGGGTG95750.05.72925625-29
AACACAG103950.05.41580110-14
AGCGACC110900.05.349285615-19
Produced by FastQC (version 0.11.5)