FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005183644

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005183644
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14042978
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGTGGT72200.020.3433844
AGTGGTA77450.019.1494335
AGCAGTG99800.015.2984852
GTGGTAT105250.014.3651076
TGGTATC113550.013.1248667
GGTATCA122550.012.6309738
GTATCAA120200.012.3388429
AAGCAGT126700.012.2763911
GCAGTGG126550.011.4956183
Produced by FastQC (version 0.11.5)