FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005183646

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005183646
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15082563
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGAGT43950.029.80551
TAAGGGG50650.025.7265789
GTGAGTA53900.024.3152792
TAGCATG56950.023.007038
CCTTTAG98000.013.9576834
ATAAGGG98850.013.4734238
GAGTATA120600.011.2823684
CTAGGAG25900.011.11840650-54
TGAGTAT125550.011.0101533
TTAGCAT128050.010.5696647
GTATAAG132450.010.38166
CTTTAGC144850.09.7414125
TATAAGG146900.09.3604017
TTTAGCA172800.08.290726
AGCATGA167000.08.1906819
TTCTAGG37200.07.818451450-54
GACCCTA39450.07.336871685-89
TCTAGGA42950.06.87231450-54
GTTCCTT184650.06.8198791
TCCTTTA208350.06.7382933
Produced by FastQC (version 0.11.5)