FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005183664

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005183664
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15098823
Sequences flagged as poor quality0
Sequence length150
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGGTA65800.022.7586255
CAGTGGT65500.022.5343764
AGCAGTG95650.016.188422
GTGGTAT97600.015.2697016
TGGTATC114250.013.3594837
GCAGTGG114500.012.9572463
GTATCAA114250.012.9183689
AAGCAGT127050.012.6981151
GGTATCA120050.012.3542128
Produced by FastQC (version 0.11.5)