FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005183674

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005183674
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15739206
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTC162040.10295309687159569No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGAGT32850.012.7806381
TGAGTAG31800.012.6853043
GTGAGTA37550.012.4694162
TAGAACC55000.08.2466527
GAGTAGA53700.07.64501574
GTTACCC52450.07.1765841
GGGGACT59200.06.2360331
CCCCATA61950.05.92717845
ACTCGCA53400.05.7975745
CTTCATT164050.05.69211151
GGGACTC63900.05.63653042
TTTCGCA175150.05.3846937
TTACCCC65600.05.3806522
TGACTCA31250.05.2988787100-104
AGTAGAA98500.05.2627825
TTCGCAG173000.05.1603058
TCGCAGA184700.05.0672969
Produced by FastQC (version 0.11.5)