FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005183675

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005183675
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15739206
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGAGTAG33400.014.2250923
AGTGAGT34850.013.5371961
GTGAGTA40250.012.7024672
TAGAACC60050.08.6313727
GAGTAGA60500.07.61533644
CTTCATT161900.06.00725561
GTTACCC54200.05.89211941
AGAACCC97450.05.6142558
TTACCCC68100.05.3928542
CCCCATA60600.05.34562975
ACTCGCA53401.8189894E-125.2575375
TACCCCA78250.05.1518273
GAACCCC110650.05.0747339
TGACTCA34100.05.066884100-104
TTCGCAG172100.05.0195038
GGGGACT59400.05.0097441
Produced by FastQC (version 0.11.5)