FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005183686

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005183686
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13927291
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCACTCA42700.025.4566659
GCCACTC60750.018.248168
AGCCACT88100.012.7466157
GATCTAG6153.311663E-511.7100363
AAGCCAC95900.011.634816
ACAAAGC133000.08.9344063
TACAAAG149050.07.73066142
ATACAAA177900.07.1598841
CCCTTAA78950.06.7469684
AAAGCCA193100.06.4862855
ACACACT41700.05.766253550-54
CCCCTAA44750.05.6624403100-104
CAAAGCC248900.05.2635114
TACACAC37950.05.23620745-49
GCCACTA50250.05.129067425-29
Produced by FastQC (version 0.11.5)