FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005183691

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005183691
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17050249
Sequences flagged as poor quality0
Sequence length150
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTAGGG11200.046.275438
CTAGGGT11600.044.6812979
TGCTAGG15850.032.699267
CTGCTAG16700.031.0347446
GGTTCTA54150.025.602521
GCTTAGT22700.019.0265527
CTTAGTG24850.017.6701168
TTAGTGG28750.016.2752179
GTTCTAT95350.015.1043912
TCTATGG94800.014.8069654
CGCTTAG30000.014.6366176
CTTGTAC112550.014.1881251
CTAGTCT14200.012.77575720-24
GATCTAG7359.719224E-812.7319383
TTCTATG125700.011.3961013
CCTAGGA15700.011.372321130-134
CTATGGC130800.010.8415325
ACTAGTC16350.010.8315820-24
GTACCGA84050.010.5343374
ACCCTAG13250.010.323154120-124
Produced by FastQC (version 0.11.5)