FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005183706

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005183706
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14051288
Sequences flagged as poor quality0
Sequence length150
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCACTA45950.019.1103788
CACCACT66550.013.95404157
CCACTAC75300.013.2865819
CCTAGAC15450.012.11504120-124
CTAGACC17000.011.264521120-124
ATACAAC92850.010.96221
ACACCAC95250.010.4297156
ACAACAC97800.09.6541393
ACGTTAA35850.08.8353124
ATCCTAG23100.08.789011115-119
AACACCA120150.08.3281665
TACAACA121900.08.2776182
TACGTTA45800.07.8683853
TCCTAGA28350.07.3645773115-119
CGTTAAA48300.07.30310065
GTTACGT45500.07.1903951
CTAACAC27450.07.08128825-29
CCCCTAA28500.06.92045125-129
ATCTAAC27700.06.757473525-29
CAACACC157650.06.66679674
Produced by FastQC (version 0.11.5)