FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005183707

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005183707
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14051288
Sequences flagged as poor quality0
Sequence length150
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCACTA47900.018.1849548
CACCACT64600.013.14934357
CCACTAC75850.012.0534259
ACGTTAA32350.011.7962164
CCTAGAC15100.010.678932120-124
ATACAAC93550.09.3033961
ACACCAC96800.09.14711956
CTAGACC18500.09.027615120-124
ACAACAC96850.08.9955333
TACGTTA44400.08.5947673
GTTACGT49000.08.5798971
CGTTAAA48400.07.73362735
TACAACA116700.07.53282642
AACACCA125100.07.4226275
ATCCTAG25050.06.840281115-119
CTAACAC28450.06.730210325-29
CTATAGC29100.06.481242715-19
CCCCTAA29650.06.458224125-129
ATCTAAC28600.06.392885725-29
TTACGTT75050.06.2406772
Produced by FastQC (version 0.11.5)