FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005183712

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005183712
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17118538
Sequences flagged as poor quality0
Sequence length150
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGGACACATCTCGTAT197380.11530190253396638TruSeq Adapter, Index 7 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGGTA99250.038.5703965
CAGTGGT113850.033.3792574
GTGGTAT136550.028.3499416
AGCAGTG142850.027.3118212
GTATCAA147650.026.8521949
GGTATCA169650.022.988318
TGGTATC173550.022.7619447
AAGCAGT176050.022.2673931
GCAGTGG194200.020.089083
GATCGGA200000.018.483911
GAGCACA200350.017.957359
TCGGAAG206350.017.8248733
AGAGCAC204300.017.7158188
ATCGGAA246450.015.1005692
AAGAGCA279250.013.42474657
GGAAGAG326050.011.63052755
CGGAAGA322800.011.3267664
GAAGAGC351250.010.7343696
AGTCACA78650.09.47273525-29
CAGTCAC96400.07.93781725-29
Produced by FastQC (version 0.11.5)