FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005183713

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005183713
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17118538
Sequences flagged as poor quality0
Sequence length150
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAGACGGAGTGTAGATCT206820.12081639214750697Illumina Single End PCR Primer 1 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCTCAA139700.023.3132767
CTCAAAC146200.022.3211679
ATGGCTC152100.020.9862985
TGGCTCA182800.017.7377136
GATCGGA207550.017.6091061
TCTGATG183400.017.0125641
TCGGAAG213050.016.8762723
GCTCAAA193450.016.7187218
GATGGCT204100.015.6748194
ATCGGAA247600.014.9324882
AGAGCGT254050.014.2617968
TTAGACG58750.012.55035530-34
GTTAGAC58200.012.39677330-34
AAGAGCG301500.012.3317217
GTGTTAG60750.012.0850825-29
GAGCGTC310050.011.9193269
CTGATGG292050.011.1050572
CGGAAGA334500.011.0505484
CCTCTAG21400.010.83547450-54
GGAAGAG341950.010.809765
Produced by FastQC (version 0.11.5)