FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005183716

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005183716
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15656530
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGTGGT72000.018.6995854
AGTGGTA81450.016.7062265
AGCAGTG107100.013.2472
GCAGTGG122200.011.9047313
GTGGTAT120900.011.6122386
GTATCAA135700.010.664099
GGTATCA141500.010.0234548
TGGTATC136400.09.9759687
AAGCAGT150200.09.7779321
AGTGAGT34902.910383E-116.2189451
GTGAGTA37957.2759576E-126.07265952
TAAGGGG35202.6193447E-105.93147859
Produced by FastQC (version 0.11.5)